狐狸Agouti基因SNPs筛查及其与毛色关联性分析

doi:10.11843/j.issn.0366-6964.2015.09.028

Abstract

Abstract:

In present study，to investigate the relationship between polymorphism of Agouti gene and coat color in fox，the whole exon 2，whole intron 2 and partial exon 3 sequence of the Agouti gene were sequenced from 58 fox samples，belonging to 5 species of 2 genera.A total of 6 SNPs(g.269G>T，g.295C>T，g.325T>G，g.518G>A，g.608C>A and g.846G>A) in intron 2 were detected firstly，revealing high genetic diversity.All individuals(n=25) of Vulpes vulpe were homozygous at the 6 sites，and the genotype were in order of GG，CC，TT，GG，CC and GG.All individuals(n=33) Alopex lagopus were another homozygous at the 6 sites，and the genotype were in order of TT，TT，GG，AA，AA and AA.Total 2 haplotypes(H1：GCTGCG and H2：TTGAAA) were found at the 6 mutation sites and their frequencies were 42.1% and 57.9%，respectively.H1 was widely distributed in all tested individuals of Vulpes vulpes，but H2 was widely distributed in all tested individuals of Alopex lagopus.Further analysis showed that the detected SNPs and haplotypes were not significant relation to coat color，but speculated that they may be the important functional sites and haplotypes to distinguish Vulpes vulpe and Alopex lagopus.

CLC Number:

S813.3

LI Ying-jie，LIU Zheng-zhu，GONG Yuan-fang，XU Gui-li，ZHANG Lei，TANG Jia-ming，DUAN Ling-xin，LIU Xie-rong，GUO Xiu-ling，LU Qi-yu. SNPs Detection of Agouti Gene and Its Associations with Coat Color in Fox[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1692-1696.

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SNPs Detection of Agouti Gene and Its Associations with Coat Color in Fox

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